《植物生理学报》 2012, 48(4): 397-402

不同阴离子对二色补血草盐腺Na⁺分泌速率的影响

杨剑超¹, 丁烽^1,2, 吴蕊蕊¹, 袁芳¹, 王宝山^1,*

山东师范大学生命科学学院逆境植物重点实验室, 济南250014; 2山东轻工业学院食品与生物工程学院, 济南250355

通信作者：王宝山；E-mail: bswang@sdnu.edu.cn；Tel: 0535-86180197

摘　要：

本文以二色补血草(Limonium bicolor)为实验材料, 用Hoagland营养液和200 mmol·L-1 NaCl、NaBr、NaNO3溶液分别处理12 h, 测定二色补血草盐腺的Na+分泌速率、叶片Na+含量和MDA (丙二醛)含量以及质膜透性, 并利用非损伤微测技术探索可能与盐腺相关的转运蛋白, 以探讨不同阴离子对二色补血草盐腺分泌Na+的作用及其可能原因。结果表明: 在NaCl处理1 2 h时二色补血草叶片Na+分泌速率达到最大, 然后逐渐下降; 不同钠盐处理下叶片Na+分泌速率为NaCl>NaBr=NaNO3>Hoagland, 而叶片Na+含量为NaBr>NaCl>NaNO3>Hoagland; 不同盐处理下叶片质膜透性和MDA含量无显著性差异; 利用Na-K-Cl共转运体专一性抑制剂bumetanide处理发现Na+分泌速率显著降低。这些结果表明Na-K-Cl共转运体可能参与盐腺分泌Na+。

关键词：阴离子; 二色补血草; 盐腺; Na+分泌; 非损伤微测技术

收稿：2012-02-06   修定：2012-03-08

资助：国家自然科学基金(30870138, 31070158)和国家支撑计划项目(2009BADA7B05)。

Effects of Different Anions on Na⁺ Secretion Rate of Salt Glands in the Leaves of Limonium bicolor

YANG Jian-Chao¹, DING Feng^1,2, WU Rui-Rui¹, YUAN Fang¹, WANG Bao-Shan^1,*

¹Key Lab of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan 250014, China; ²School of Food and Bioengineering, Shandong Polytechnic University, Jinan 250355, China

Corresponding author: WANG Bao-Shan; E-mail: bswang@sdnu.edu.cn; Tel: 0535-86180197

Abstract:

Exo-recretohalophyte Limonium bicolor was treated with Hoagland’s solution (control), 200 mmol·L^-1 NaCl, NaBr and NaNO₃ for 12 h, respectively. In order to examine the effects of different anions on Na⁺ secretion ability and to elucidate possible reasons, the salt secretion rate of salt glands, Na⁺ content, MDA content, and the plasma membrane permeability were determined. Plasmalemma transporters such as Na-K-Cl co-transporter was investigated using the Scanning Ion-selective Electrode Technique (SIET). The results showed that Na⁺ secretion rates of salt glands in Limonium bicolor leaves significantly increased with time and reached the highest values at 12 h and then gradually declined. Na⁺ secretion rates of salt glands in Limonium bicolor leaves under different salt treatments were NaCl>NaBr=NaNO₃>Hoagland, but Na⁺ contents in leaves were NaBr>NaCl>NaNO₃>Hoagland. Moreover, there were no significant differences in leaf plasma membrane damage and MDA contents among different treatments. The Na⁺ secretion rate significantly reduced by treating salt glands with Na-K-Cl co-transporter inhibitor bumetanide. These results suggested that the Na-K-Cl cotransporter is possibly involved in the process of Na⁺ secretion of salt glands.

Key words: anions; Limonium bicolor; salt glands; Na⁺ secretion; Scanning Ion-selective Electrode Technique (SIET)